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Updated: Jun 7, 2025

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation
Published on: December 9, 2021
Systems-level reconstruction of kinase phosphosignaling networks regulating endothelial barrier integrity using
Ling Wei1, John D Aitchison2,3,4, Alexis Kaushansky2,3,5
1Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, WA, 98109, USA. ling.wei@seattlechildrens.org.
None:
Phosphosignaling networks control cellular processes. We built kinase-mediated regulatory networks elicited by thrombin stimulation of brain endothelial cells using two computational strategies: Temporal Pathway Synthesizer (TPS), which uses phosphoproteomics data as input, and Temporally REsolved KInase Network Generation (TREKING), which uses kinase inhibitor screens. TPS and TREKING predicted overlapping barrier-regulatory kinases connected with unique network topology. Each strategy effectively describes regulatory signaling networks and is broadly applicable across biological systems.
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