Isolation, resolution and partial characterization of two Robinia pseudoacacia seed lectins

Two lectins from the seeds of Robinia pseudoacacia were isolated and resolved in a single affinity chromatography run using immobilized ovomucoid by successive elution with different buffers. The lectins were characterized by their subunit sizes, total molecular masses and isoelectric points as well as by their ability to bind to ConA-Sepharose and to stimulate mitosis in murine lymphocytes.