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Review and Practical Guide for Getting Started With Single-Cell Proteomics
Hsien-Jung L Lin1, Kei G I Webber1, Andikan J Nwosu1
1Department of Chemistry & Biochemistry, Brigham Young University, Provo, Utah, USA.
Proteomics
|November 16, 2024
Summary
Single-cell proteomics (SCP) is now accessible to more labs with new tools. This guide offers practical steps for researchers to perform SCP experiments and achieve meaningful single-cell discoveries.
Area of Science:
- Proteomics
- Biotechnology
- Analytical Chemistry
Background:
- Single-cell proteomics (SCP) has seen significant advancements with new commercial tools.
- The field is now mature and accessible to labs performing bulk proteomics.
Purpose of the Study:
- To provide a practical guide for new researchers entering the SCP field.
- To highlight recent work that has lowered the barrier to entry for SCP.
Main Methods:
- Outlining fundamental principles of SCP experiments.
- Detailing sample preparation, separation, and mass spectrometry data acquisition and analysis.
- Recommending label-free SCP workflows for straightforward, accurate results.
Main Results:
- Recent advancements have made SCP more accessible.
- Label-free workflows are recommended for ease of use and accuracy.
- Researchers can confidently perform SCP experiments.
Conclusions:
- The SCP field is broadly accessible to researchers with basic capabilities.
- Practical guidance and accessible methods empower new researchers in SCP.
- SCP enables meaningful discoveries at the single-cell level.
