Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Optimization of trap column properties and loading conditions for proteome profiling of single-cell-level sample inputs.

Analytical and bioanalytical chemistry·2026

Same author

Modification of a Low-Cost Pipetting Robot for Nanoliter Liquid Handling and Autosampling for Liquid Chromatography-Mass Spectrometry.

Journal of separation science·2026

Same author

Evaluation and application of chemical decrosslinking in the context of histopathological spatial proteomics.

bioRxiv : the preprint server for biology·2026

Same author

CMG2 interaction with actin is required for growth factor-induced chemotaxis in endothelial cells.

bioRxiv : the preprint server for biology·2025

Same author

The $10 proteome: low-cost, deep and quantitative proteome profiling of limited sample amounts using the Orbitrap Astral and timsTOF Ultra 2 mass spectrometers.

bioRxiv : the preprint server for biology·2025

Same author

Proteomic and Metabolomic Data Transparency.

Journal of proteome research·2025

Same journal

Light-Induced Proteomic Changes in Pseudomonas aeruginosa Biofilms.

Proteomics·2026

Same journal

Decade-Resolved Proteomic Profiling of Gastric Cancer FFPE Archives: Evaluating Storage-Associated Shifts and Signal Stability Over 50 Years.

Proteomics·2026

Same journal

Proteome-Scale Mining of Metal-Associated Proteins of Monkeypox Virus.

Proteomics·2026

Same journal

Optimized Sample Handling Minimizes Peptide Adsorption to Plastics to Enable High Sensitivity Evosep Based Chemical Proteomics.

Proteomics·2026

Same journal

Toward Predicting Pandemic Potential: A Comparative Analysis of Virus-Host Interactions Between Diverse Influenza A Viruses and the Human Innate Immune System.

Proteomics·2026

Same journal

Functional Divergence of Mucus in Pacific Oyster (Crassostrea gigas): Insights From Integrated Proteomic and Rheological Study.

Proteomics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 7, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

Review and Practical Guide for Getting Started With Single-Cell Proteomics.

Hsien-Jung L Lin¹, Kei G I Webber¹, Andikan J Nwosu¹

¹Department of Chemistry & Biochemistry, Brigham Young University, Provo, Utah, USA.

|November 16, 2024

Summary

This summary is machine-generated.

Single-cell proteomics (SCP) is now accessible to more labs with new tools. This guide offers practical steps for researchers to perform SCP experiments and achieve meaningful single-cell discoveries.

Keywords:

cellular heterogeneity data acquisition methods liquid handling automation sample preparation single‐cell proteomics (SCP)

More Related Videos

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Published on: April 21, 2022

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Related Experiment Videos

Last Updated: Jun 7, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Published on: April 21, 2022

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Area of Science:

Proteomics
Biotechnology
Analytical Chemistry

Background:

Single-cell proteomics (SCP) has seen significant advancements with new commercial tools.
The field is now mature and accessible to labs performing bulk proteomics.

Purpose of the Study:

To provide a practical guide for new researchers entering the SCP field.
To highlight recent work that has lowered the barrier to entry for SCP.

Main Methods:

Outlining fundamental principles of SCP experiments.
Detailing sample preparation, separation, and mass spectrometry data acquisition and analysis.
Recommending label-free SCP workflows for straightforward, accurate results.

Main Results:

Recent advancements have made SCP more accessible.
Label-free workflows are recommended for ease of use and accuracy.
Researchers can confidently perform SCP experiments.

Conclusions:

The SCP field is broadly accessible to researchers with basic capabilities.
Practical guidance and accessible methods empower new researchers in SCP.
SCP enables meaningful discoveries at the single-cell level.