Detection of Salmonella enterica in food using targeted mass spectrometry

The high prevalence of Salmonella enterica necessitates rapid and efficient detection methods. Targeted mass spectrometry (MS) using multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) has become a promising technique with improved specificity and sensitivity. We develop a novel targeted MS method for detecting S. enterica in food based on peptide biomarkers. Using a combination of four peptide biomarkers, this newly developed method could accurately distinguish S. enterica from other conventional food-borne pathogens. When combined with buoyant density centrifugation (BDC), Salmonella was efficiently separated from food matrices. Based on this discovery, this method was successfully applied to detect S. enterica in both artificially and naturally contaminated food samples, comparable to the culture method. These results demonstrate the potential of the targeted MS method in various food categories and are expected to be an alternative approach for S. enterica detection in food.