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Updated: Jun 7, 2025

Sample Preparation Method of Scanning and Transmission Electron Microscope for the Appendages of Woodboring Beetle
Published on: February 3, 2020
A histological procedure for the study of insect chitinized tissues in light microscopy
Manuela Gallardo1, María Valladolid2
1Laboratory of Histology, Museo Nacional de Ciencias Naturales (MNCN-CSIC), c/ José Gutiérrez Abascal, 2, Madrid 28006, Spain.
Abstract:
We present a procedure to obtain histological preparations of insect cuticles for their observation in ligth microscopy. The use of EDTA (ethylendiaminetetraacetic acid), as a chelator of certain metals present in chitin, soften the tissues and allows us to cut them. The tissues were embbeding in plastic resines in two different protocols and cutted by cryostat (20-30 μm) and ultramicrotome (4 μm) respectively. This procedure, based on traditional histology products, allows us to obtain preparations observable under an optical microscope, facilitating the study of the internal structure of the cuticule, e.g. visualizing the different layers or structures such as glands or setae.

