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Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: Jun 7, 2025

Detection of Bacteria Using Fluorogenic DNAzymes

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

Aptamer and DNAzyme Based Colorimetric Biosensors for Pathogen Detection.

Rudi Liu¹, Jiuxing Li¹, Bruno J Salena²

¹Department of Biochemistry and Biomedical Sciences, Michael G. DeGroote Institute of Infectious Disease Research, School of Biomedical Engineering, Biointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada.

Angewandte Chemie (International Ed. in English)

|November 17, 2024

Summary

Functional nucleic acids (FNAs), like aptamers and DNAzymes, offer precise pathogen detection. Integrating FNAs into colorimetric biosensors creates simple, cost-effective diagnostic tools for health and safety.

Keywords:

Aptamer Bacteria Colorimetric Biosensors DNAzyme Viruses

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Last Updated: Jun 7, 2025

Detection of Bacteria Using Fluorogenic DNAzymes

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

Colorimetric Detection of Bacteria Using Litmus Test

Colorimetric Detection of Bacteria Using Litmus Test

Published on: September 17, 2016

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

Area of Science:

Molecular Biology
Biotechnology
Analytical Chemistry

Background:

Pathogen detection is crucial for public health, environmental safety, and food security.
Functional nucleic acids (FNAs), including aptamers and DNAzymes, are highly specific molecular tools for detecting disease-causing agents.
Existing detection methods face challenges in speed, cost, and accessibility.

Purpose of the Study:

To review the in vitro selection of functional nucleic acids (FNAs) for pathogen detection.
To explore the integration of FNAs into colorimetric biosensors for diagnostics.
To highlight advancements and future directions in FNA-based biosensing.

Main Methods:

In vitro selection (SELEX) for aptamers targeting specific biomarkers and whole pathogens (bacteria, viruses).
Selection of DNAzymes for bacterial detection.
Development and analysis of colorimetric biosensors utilizing FNAs.

Main Results:

FNAs demonstrate high specificity and affinity for various pathogens.
Colorimetric biosensors based on FNAs, including aptamers and DNAzymes, enable visible, cost-effective pathogen detection.
Diverse colorimetric strategies (e.g., gold nanoparticles, G-quadruplexes, nanozymes) enhance biosensor performance.

Conclusions:

FNA-based colorimetric biosensors represent a promising technology for rapid and accessible pathogen detection.
Further development is needed to address current challenges and optimize these biosensing platforms.
These advancements hold significant potential for point-of-care diagnostics and public health surveillance.