The effect of AH26, Endoseal and ZOE sealers on Candida albicans and Enterococcus faecalis

In the oral cavity, root canal treatment failure (endodontics) is often due to the persistence of microorganisms in the root canal system after treatment, or re-infection of the root due to insufficient flooding. In addition, microorganisms are essential for the development of peri radicular diseases and are one of the main factors related to root canal treatment failure. Microorganisms that are commonly isolated from teeth that have failed endodontic treatment include Enterococcus (E.) faecalis and Candida (C.) albicans. Bacterial infection is closely related to the failure of root canal treatment, and the use of root sealer with antimicrobial activity and biological compatibility is necessary for the success of root canal treatment. One of the main goals of endodontic treatment is to eliminate intracanal infection and root canal flooding in order to prevent re-infection. Therefore, the aim of the current study is to evaluate the antibacterial and antifungal activity of ZOE, AH26 and Endoseal sealers in vitro.

To study the effect of each sealer; AH26, ZOE and Endoseal on E. faecalis and C. albicans, 10 samples were considered. Firstly, the freshly prepared sealers were poured inside the microtube with nutrient broth and then 10 µl of E. faecalis and C. albicans suspensions were separately added into a microtube and were kept 24 hours in the incubator to grow.

The data were analyzed with Kolmogrov-Smirnov test and SPSS Version 19. Our results demonstrated that the resistance rate of C. albicans to ZOE, Endoseal and AH26 sealers was 0%, 100% and 70%, respectively. These values for the E. faecalis was 80%, 100% and 40% respectively. The resistance was defined when the microorganism was growth in subculture in LB agar.

ZOE sealer has highest antimicrobial effect after that AH26 sealer and the least antimicrobial effect is related to Endoseal sealer.