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Related Experiment Video

Updated: Jun 7, 2025

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification

Published on: November 1, 2024

Application of DNA Barcoding to Identify Medicinal Plants.

Shuai Yang¹, Shilin Chen¹, Jing Mo²

¹School of Pharmacy, Guizhou University of Traditional Chinese Medicine; School of Pharmacy/School of Modern Chinese Medicine Industry, Chengdu University of Traditional Chinese Medicine.

Journal of Visualized Experiments : Jove

|November 18, 2024

Summary

DNA barcoding accurately identifies medicinal plants and detects adulterants using the internal transcribed spacer 2 (ITS2) region. This method ensures the quality and authenticity of traditional plant-based medicines.

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Area of Science:

Botany
Genetics
Pharmacognosy

Background:

Medicinal plants are vital globally but face challenges from adulteration.
Accurate identification of these plants is crucial for health and disease treatment.

Purpose of the Study:

To establish a DNA barcoding protocol for reliable identification of medicinal plants.
To differentiate genuine medicinal plants from adulterants.

Main Methods:

DNA extraction using the centrifugal column method.
Amplification of the internal transcribed spacer 2 (ITS2) region with universal plant primers.
Sanger sequencing, sequence alignment, and phylogenetic analysis using a neighbor-joining tree.

Main Results:

Universal primers successfully amplified the target ITS2 region.
Basic Local Alignment Search Tool (BLAST) achieved 100% identification accuracy.
Phylogenetic analysis confirmed sequences clustered with known species (A. sinensis), with 100% clade support.

Conclusions:

The developed DNA barcoding protocol is effective for identifying medicinal plants.
This method provides a reliable approach to detect adulterants in herbal products.
The study offers a reference for quality control in traditional medicine.