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A Microfluidic Platform for Stimulating Chondrocytes with Dynamic Compression
Published on: September 13, 2019
Application of hydrostatic pressure up-regulates Sost gene expression in osteocytic spheroids
Jeonghyun Kim1, Kotone Niioka1, Eijiro Maeda1
1Department of Mechanical Systems Engineering, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya, Japan.
Abstract:
In this study, we developed a hydrostatic pressurizing chamber capable of applying hydrostatic pressure to osteocytic spheroids derived from mouse osteoblastic MC3T3-E1 cells. Our results demonstrate that a 4-h exposure to 200 kPa of hydrostatic pressure did not alter the apparent morphology of the spheroids. However, gene expression analysis revealed a significant up-regulation of Sost, a marker of late-stage osteocyte differentiation.
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