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Updated: Jun 7, 2025

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A novel microfluidic chip for on-site radiation risk evaluation.

Kenta Takahashi1, Takahiro Tamura2, Kosuke Yamada2

  • 1Department of Biological Sciences, Ibaraki University, Mito, Japan. asako.nakamura.wasabi@vc.ibaraki.ac.jp.

The Analyst
|November 20, 2024
PubMed
Summary

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Tempol Exerts Radioprotective Effects by Suppressing Radiation-Induced DNA Double-Strand Break Formation.

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This study introduces a microfluidic chip for rapid, on-site radiation risk assessment. The device simplifies the DNA double-strand break (DSB) assay, enabling biological effect evaluation of radiation exposure outside conventional lab settings.

Area of Science:

  • Biomedical Engineering
  • Radiation Biology
  • Microfluidics

Background:

  • Conventional DNA double-strand break (DSB) assays require extensive laboratory space and complex procedures.
  • On-site biological effect evaluation of radiation exposure is crucial for rapid risk assessment.

Purpose of the Study:

  • To develop a microfluidic chip for on-site radiation risk evaluation.
  • To simplify the DNA double-strand break (DSB) assay using immunofluorescence staining for phosphorylated histone, H2AX (γ-H2AX).

Main Methods:

  • A microfluidic chip was designed to separate and trap lymphocytes from whole blood using size-based separation and a unique trap structure.
  • The γ-H2AX assay was performed directly on the chip.
  • Bead experiments and cell-based assays using human lymphoblastoid TK6 cells were conducted to validate the chip's performance.

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Related Experiment Videos

Last Updated: Jun 7, 2025

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Main Results:

  • The chip successfully separated beads of different sizes (10 μm and 27 μm) using centrifugal force and passive structures.
  • Over 95% of 10 μm beads were trapped in the lymphocyte trap structures (LTSs).
  • TK6 cells were effectively trapped, and γ-H2AX foci, indicating DSBs, were observed on the chip.

Conclusions:

  • The proposed microfluidic chip simplifies the γ-H2AX assay protocol.
  • This novel method enables on-site biological effect evaluation of radiation exposure, overcoming the limitations of conventional laboratory-based assays.