Evaluation of mycotoxin binders against deoxynivalenol and fumonisin B₁ using isotherm models and linear equations

This study was conducted to evaluate the adsorbent characteristics of two mycotoxin binders (BBAc - composed of bentonite, β-glucans, and activated charcoal, and SepHt - composed of heat-treated sepiolite) against deoxynivalenol (DON) and fumonisin B₁ (FB₁) using Brunaeur-Emmett-Teller, Dubinin-Radushkevich, Freundlich, and Langmuir isotherm models and linear regression equations. Both products were tested in vitro at 0.5% with increasing levels of DON or FB₁ (0.5-10 mg L^-1) using solutions of pH 3 and pH 6 and analysed by LC-MS/MS. FB₁ adsorption rates were not different between the products (p > 0.05) at pH 3 and pH 6. At a DON concentration of 1 mg L^-1, BBAc had higher (p < 0.05) adsorption rates than SepHt. For DON, the Freundlich model had the best fit with BBAc at pH 3 and 6, and the Langmuir model with SepHt at both pHs. For FB₁, the Freundlich model had the best fit with BBAc and SepHt at pH 3, and the Langmuir model with both products at pH 6. All the linear regression equations had lower R² than the isotherm models. Therefore, the adsorption isotherm models provided more informative and reliable data for the mycotoxins and mycotoxin binders tested herein.