A Rhodamine-based high-sensitivity low-cytotoxicity probe for rapid turn-on detection of Hg²

By integrating Rhodamine B and 4-phenylmorpholine moieties, a novel fluorescent probe named RhPy is synthesized for detecting Hg²⁺. Its recognition mechanism involves the reaction of Hg²⁺ with dithiooxamide, ultimately triggering the opening of the Rhodamine spirolactam and forming a new molecule RhPy-S with strong emission. The probe exhibits impressive limit of detection (0.015 μM) and short response time (<10 s). Importantly, RhPy shows almost none-cytotoxicity and RhPy-S has the emission spectrum peaking at 596 nm, which endow the probe with a good tissue penetration ability and practical utility in living cells, zebrafish and in vivo mice models. This work advances the field by providing a highly sensitive chemosensor for both environmental and biological applications.