Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitochondria01:37

Mitochondria

11.1K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
11.1K
Mitochondrial Membranes01:45

Mitochondrial Membranes

8.7K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

LOESS and DE-SWAN can induce artifactual "waves" of molecular aging.

bioRxiv : the preprint server for biology·2026
Same author

Robust discovery of mutational signatures using power posteriors.

PLoS computational biology·2026
Same author

Do you want to live forever? Lessons learned from the biology of aging.

PLoS biology·2026
Same author

Peroxisomes orchestrate metabolic flexibility and longevity via an interorganelle cascade.

Nature aging·2026
Same author

Local graph estimation with pathwise false discovery control.

Nature communications·2026
Same author

The problem with one-size-fits-all medicine: Biological sex and the aging immune system.

PLoS biology·2026

Related Experiment Video

Updated: Jun 6, 2025

Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging
05:29

Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging

Published on: January 17, 2025

791

Endogenous mitochondrial NAD(P)H fluorescence can predict lifespan.

Christopher S Morrow1, Pallas Yao1, Carlos A Vergani-Junior1,2

  • 1Department of Molecular Metabolism, Harvard TH Chan School of Public Health, Boston, MA, USA.

Communications Biology
|November 21, 2024
PubMed
Summary

Researchers developed novel "mito-NAD(P)H age clocks" using non-destructive imaging to measure mitochondrial changes. These clocks accurately predict biological age and lifespan by analyzing nicotinamide adenine dinucleotide phosphate (NAD(P)H) fluorescence in mitochondria.

More Related Videos

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
12:22

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Published on: July 22, 2013

21.0K
Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis
09:47

Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis

Published on: June 2, 2023

2.1K

Related Experiment Videos

Last Updated: Jun 6, 2025

Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging
05:29

Imaging and Quantifying Mitochondrial Morphology in C. elegans During Aging

Published on: January 17, 2025

791
Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
12:22

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Published on: July 22, 2013

21.0K
Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis
09:47

Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis

Published on: June 2, 2023

2.1K

Area of Science:

  • Biophysics
  • Cellular Biology
  • Gerontology

Background:

  • Aging clocks are crucial for predicting health outcomes and understanding aging heterogeneity.
  • Existing methods face limitations like low resolution, long processing times, sample destruction, and phenotype bias.

Purpose of the Study:

  • To introduce a non-destructive, label-free, subcellular resolution method for aging quantification.
  • To develop novel aging prediction models based on mitochondrial biophysical properties.

Main Methods:

  • Utilized fluorescence lifetime imaging (FLIM) of endogenous NAD(P)H fluorescence.
  • Quantified age-dependent biophysical changes in mitochondrial NAD(P)H.
  • Constructed cellular resolution aging prediction models ('mito-NAD(P)H age clocks').

Main Results:

  • Uncovered age-dependent changes in mitochondrial NAD(P)H across tissues in C. elegans, linked to physiological decline.
  • Mito-NAD(P)H age clocks accurately predicted age, resolved aging rate heterogeneity, and estimated remaining lifespan.
  • Revealed spatiotemporal asynchrony in mitochondrial aging and identified attenuated changes associated with longevity.

Conclusions:

  • Mitochondrial NAD(P)H FLIM offers a high-resolution, non-destructive approach to aging research.
  • Mito-NAD(P)H age clocks provide a powerful new tool for quantifying aging and its heterogeneity.
  • This method enhances understanding of mitochondrial aging mechanisms and expands aging assessment capabilities.