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A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

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Near-Infrared Bioimaging Using Two-photon Fluorescent Probes.

Pragti¹, Bidyut Kumar Kundu¹, Rui Chen¹

¹Department of Chemistry, University of Cincinnati, Cincinnati, OH, 45221, USA.

Advanced Healthcare Materials

|November 22, 2024

Summary

Two-photon absorption (TPA) fluorescent probes offer superior near-infrared (NIR) bioimaging by enhancing tissue penetration and reducing photodamage. Future advancements focus on in vivo applications and dual-function probes for diagnosis and therapy.

Keywords:

bioimaging fluorescent probes two‐photon absorption

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In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy

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Area of Science:

Biomedical Imaging
Optics
Materials Science

Background:

Near-infrared (NIR) bioimaging is a key technology in biomedical research.
Two-photon absorption (TPA) fluorescent probes offer advantages over one-photon absorption (OPA) probes.
TPA probes provide enhanced tissue penetration, reduced photodamage, and improved spatial resolution.

Purpose of the Study:

To provide a comprehensive overview of TPA fluorescent probes for NIR bioimaging.
To discuss fundamental principles, design strategies, and applications of TPA probes.
To highlight recent advancements and future perspectives in TPA probe development.

Main Methods:

Review of molecular fluorescent probes, including organic, inorganic, and COF/MOF-based systems.
Discussion of stimulated TPA probes responsive to environmental factors (pH, redox, enzymes, hypoxia).
Analysis of design strategies for TPA probe development.

Main Results:

TPA probes demonstrate significant potential for advanced bioimaging applications.
Various molecular designs enable tailored TPA probe properties.
Stimulated TPA probes offer dynamic and responsive imaging capabilities.

Conclusions:

TPA fluorescent probes are crucial for next-generation NIR bioimaging.
Further research should focus on in vivo studies and dual-function probes.
Development of TPA probes will advance disease diagnosis and therapeutic strategies.