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Systematic review and meta-analysis of bulk RNAseq studies in human Alzheimer's disease brain tissue
Bernardo Aguzzoli Heberle1,2, Kristin L Fox2,3, Lucas Lobraico Libermann4
1Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY.
Objective:
To systematically review and meta-analyze bulk RNA sequencing studies comparing Alzheimer's disease (AD) patients with controls in human brain tissue, assessing study quality and identifying key genes and pathways.
Methods:
We searched PubMed, Web of Science, and Scopus on September 23, 2023, for studies using bulk RNAseq on primary human brain tissue from AD patients and controls. Excluded were non-primary tissue, re-analyses without new data, limited RNA types and gene panels. Quality was assessed with a 10-category tool. Meta-analysis used high-quality datasets.
Results:
From 3,266 records, 24 studies met criteria. Meta-analysis found 571 differentially expressed genes (DEGs) in temporal lobe and 189 in frontal lobe; overlapping pathways included "Tube morphogenesis" and "Neuroactive ligand-receptor interaction."
Limitations:
Study heterogeneity and limited data tables constrained the review.
Conclusions:
Rigorous methods are vital in AD transcriptomic studies. Findings enhance understanding of transcriptomic changes, aiding biomarker and therapeutic development.
Registration:
PROSPERO (CRD42023466522).
