Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vivo dedifferentiation of human epidermal cells.

Cell biology international·2007
Same author

What is in a word? No versus Yes differentially engage the lateral orbitofrontal cortex.

Emotion (Washington, D.C.)·2007
Same author

[Gene expression profile changes in oral verrucous carcinoma and oral squamous cell carcinoma].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2007
Same author

Morphology of critical nuclei in solid-state phase transformations.

Physical review letters·2007
Same author

Enhanced cooperative activation effect in the hydrolytic kinetic resolution of epoxides on [Co(salen)] catalysts confined in nanocages.

Angewandte Chemie (International ed. in English)·2007
Same author

[Construction of the three-dimensional finite element model of micro -implant -maxilla].

Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology·2007

Related Experiment Video

Updated: Jun 6, 2025

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
06:53

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Published on: July 10, 2021

5.1K

Optimization of vitrification methods for equine oocytes.

Ming Du1, Xinyu Li1, Bayinnamula1

  • 1College of Animal Science, Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Equine Research Center, Inner Mongolia Agricultural University, Hohhot 010018, China.

Tissue & Cell
|November 22, 2024
PubMed
Summary

Vitrification freezing is key for equine oocyte cryopreservation. The study optimized protocols using Ethylene glycol and Dimethyl sulfoxide, finding ED10 + EDFS30 yielded the highest maturation rate and lowest apoptosis gene expression.

Keywords:
Mongolian horseVitrification freezingoocyte

More Related Videos

Minimum Volume Vitrification of Immature Feline Oocytes
07:16

Minimum Volume Vitrification of Immature Feline Oocytes

Published on: June 24, 2020

6.4K
Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives
08:46

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Published on: September 16, 2021

5.9K

Related Experiment Videos

Last Updated: Jun 6, 2025

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
06:53

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Published on: July 10, 2021

5.1K
Minimum Volume Vitrification of Immature Feline Oocytes
07:16

Minimum Volume Vitrification of Immature Feline Oocytes

Published on: June 24, 2020

6.4K
Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives
08:46

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Published on: September 16, 2021

5.9K

Area of Science:

  • Reproductive Biology
  • Cryobiology
  • Animal Science

Background:

  • Equine germplasm preservation is crucial.
  • Vitrification is the preferred method for equine oocyte cryopreservation due to its efficiency and cost-effectiveness.
  • Optimizing cryoprotectant solutions and exposure times is essential for successful cryopreservation.

Purpose of the Study:

  • To investigate and compare various vitrification schemes for equine oocytes.
  • To determine the optimal combination of cryoprotectants and exposure times for equine oocyte cryopreservation.
  • To evaluate the effects of different vitrification protocols on oocyte maturation and apoptosis.

Main Methods:

  • Equine oocytes were exposed to equilibrium solutions containing Ethylene glycol (EG) and Dimethyl sulfoxide (DMSO) at varying concentrations and times (ED10, ED15, ED20).
  • Three cryosolution formulations (EDFS30, EDFS35, EDFS40) with different proportions of EG, DMSO, Sucrose, and Ficoll were prepared.
  • Twenty-seven different freezing protocols were tested, and the best-performing protocol (ED10 + EDFS30 + 80s) and worst (ED20 + EDFS40 + 120s) were identified.

Main Results:

  • Protocol ED10 (39s) + EDFS30 + 80s resulted in the highest in vitro culture maturation rate (19.3%).
  • Protocol ED20 (20s) + EDFS40 + 120s showed the poorest outcomes.
  • Apoptosis gene analysis indicated significantly higher expression of BAX, BID, BOK, and TP53 in the ED20 + EDFS40 group compared to the optimal group and controls (p<0.01).

Conclusions:

  • The study identified an optimal vitrification protocol (ED10 + EDFS30) for equine oocytes, balancing cryoprotectant concentration and exposure time.
  • The findings suggest that specific vitrification schemes can significantly impact equine oocyte viability and reduce apoptosis.
  • Further research into cryoprotectant toxicity and cryoinjury mechanisms is warranted for improving equine oocyte cryopreservation.