Unraveling temperature effects on caproate and caprylate production from waste activated sludge

This study explored the impact of different temperatures on the continuous production of medium-chain fatty acids (MCFAs) from waste activated sludge (WAS). Experimental results showed that there was almost no MCFAs accumulation at 55 °C. Both 25 °C and 37 °C were suitable for MCFAs production, with 25 °C favoring high-value caprylate production. The metagenomic and metatranscriptomic analysis highlighted reverse β-oxidization as the main chain elongation (CE) cycle. The lack of CE-related microorganisms and enzymes at 55 °C hindered MCFAs production, in contrast to the heightened activity observed at 25 °C and 37 °C, with peak activity at 25 °C leading to increased longer-chain MCFAs synthesis. 37 °C promoted hydrolysis and acidification, resulting in a accumulation of higher short-chain fatty acids, but further elongation to MCFAs would be hindered by product toxicity. This research concludes that 25 °C is the most effective temperature for the production of WAS-derived MCFAs, offering significant economic advantages.