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Updated: Jun 6, 2025

High-Resolution Respirometry to Assess Bioenergetics in Cells and Tissues Using Chamber- and Plate-Based Respirometers
Published on: October 26, 2021
Textbook oxidative phosphorylation needs to be rewritten
Alicia J Kowaltowski1, Fernando Abdulkader2
1Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.
Abstract:
Oxidative phosphorylation (OxPhos) is the energy-transfer process that generates most of our ATP, fueled by proton and electrical gradients across the inner mitochondrial membrane. A new surprising finding by Hernansanz-Agustín et al. demonstrates that between one-third and half of this gradient is attributable to Na+, transported in exchange for protons within complex I.
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