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Related Experiment Video

Updated: Jun 6, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Engineering cold resilience: implementing gene editing tools for plant cold stress tolerance.

Khushbu Kumari1,2, Suman Gusain1,2, Rohit Joshi3,4

  • 1Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India.

Planta
|November 23, 2024
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Summary

Innovative genome-editing tools are crucial for enhancing plant cold tolerance and understanding cold stress responses. These advanced techniques offer a path to developing climate-resilient crops for improved agricultural productivity in challenging environments.

Keywords:
CRISPR-Cas9Cold stressEpigenetic regulationGenome editingPhysiological responseTranscription factors

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Area of Science:

  • Plant Science
  • Genetics
  • Agricultural Science

Background:

  • Cold stress significantly impacts plant growth and crop productivity, especially in high-altitude regions.
  • Plants possess evolved mechanisms for low-temperature resistance, but many lack sufficient cold acclimatization.
  • Conventional breeding methods have shown limited success in improving crop cold resistance.

Purpose of the Study:

  • To highlight the need for innovative strategies to enhance plant cold tolerance.
  • To explore the potential of genome-editing tools in understanding cold stress.
  • To review advancements in gene editing for developing cold-resilient crops.

Main Methods:

  • Review of existing literature on plant cold tolerance mechanisms.
  • Discussion of various genome-editing tools: ZFNs, TALENs, CRISPR/Cas9/Cas12a, PE, and RLR.
  • Specific focus on the CRISPR/Cas system's application in plant science.

Main Results:

  • Genome-editing tools offer efficient, targeted modifications for developing cold-tolerant varieties.
  • The CRISPR/Cas system is a groundbreaking tool for genome editing across diverse species.
  • Advanced genomic approaches are essential for creating crops with improved adaptability and yield.

Conclusions:

  • Genome editing deepens the understanding of genes involved in cold stress response.
  • Targeted genetic modifications are key to developing future climate-resilient crops.
  • Innovative approaches using gene editing are vital for agricultural sustainability.