Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple method to heparinize biological materials.

Y Noishiki, T Miyata

    Journal of Biomedical Materials Research
    |March 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Aortic aneurysms in patients with autoimmune disorders treated with corticosteroids.

    European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·1995
    Same author

    Gender differences of disturbed hemostasis related to fasting insulin level in healthy very elderly Japanese aged > or = 75 years.

    Atherosclerosis·1995
    Same author

    Substance P stimulates the loss of cell-associated high molecular weight glycoconjugates from cultured hamster tracheal epithelial cells through polymorphonuclear leucocytes activation.

    Biochemistry and molecular biology international·1995
    Same author

    Activation of tissue factor-induced coagulation and endothelial cell dysfunction in non-insulin-dependent diabetic patients with microalbuminuria.

    Arteriosclerosis, thrombosis, and vascular biology·1995
    Same author

    Phylogenetic position of Dictyostelium inferred from multiple protein data sets.

    Journal of molecular evolution·1995
    Same author

    Identification of two highly homologous presynaptic proteins distinctly localized at the dendritic and somatic synapses.

    FEBS letters·1995
    Same journal

    Optimizing the tensile properties of polyvinyl alcohol hydrogel for the construction of a bioprosthetic heart valve stent.

    Journal of biomedical materials research·2002
    Same journal

    Wear of conventional and cross-linked ultra-high-molecular-weight polyethylene acetabular cups against polished and roughened CoCr femoral heads in a biaxial hip simulator.

    Journal of biomedical materials research·2002
    Same journal

    Immobilization of natural macromolecules on poly-L-lactic acid membrane surface in order to improve its cytocompatibility.

    Journal of biomedical materials research·2002
    Same journal

    Light scattering and in vitro biocompatibility studies of poly (vinyl pyrrolidone) derivatives with amino-acid-dependent groups.

    Journal of biomedical materials research·2002
    Same journal

    Immobilization of an oxalate-degrading enzyme on silicone elastomer.

    Journal of biomedical materials research·2002
    Same journal

    An evaluation of the cytotoxic effects of orthodontic bonding adhesives upon a primary human oral gingival fibroblast culture and a permanent, human oral cancer-cell line.

    Journal of biomedical materials research·2002
    See all related articles

    Researchers developed a novel method to make biologic materials antithrombogenic by binding heparin to collagen using protamine. This technique enhances vascular grafts and shows potential for artificial organs and antiadhesive membranes.

    Area of Science:

    • Biomaterials Science
    • Biomedical Engineering
    • Cardiovascular Research

    Background:

    • Biologic materials often require modification to prevent blood clot formation (thrombosis) when used in medical applications.
    • Enhancing the antithrombogenic properties of materials is crucial for the success of vascular grafts and artificial organs.
    • Existing methods for antithrombogenicity may have limitations in terms of durability or control over heparin binding.

    Purpose of the Study:

    • To develop a novel method for inducing antithrombogenicity in biologic materials.
    • To investigate the use of protamine as a linker to enhance heparin binding to collagenous substrates.
    • To evaluate the antithrombogenic efficacy of the modified material as a vascular substitute.

    Main Methods:

    • A protamine sulfate solution was applied to a collagen-rich material (porcine ureter).

    Related Experiment Videos

  • The adsorbed protamine was cross-linked to the material using glutaraldehyde.
  • Heparin was ionically bound to the collagen-protamine complex, with control over heparinization achieved by adjusting protamine fixation.
  • The modified porcine ureter was tested as a vascular substitute in experimental animal studies.

    • Main Results:

    • The developed method successfully induced antithrombogenicity in the biologic material.
    • The heparinized porcine ureter demonstrated excellent antithrombogenic performance in animal models.
    • The degree of protamine fixation allowed for controlled binding and distribution of heparin.

    Conclusions:

    • This novel method effectively imparts antithrombogenicity to biologic materials by creating a stable heparin-collagen complex.
    • The approach shows significant promise for developing antithrombogenic artificial organs and antiadhesive membranes.
    • The controlled heparinization technique offers a versatile strategy for biomaterial surface modification.