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Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

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Published on: June 1, 2012

Self-Extracting Dextran-Based Hydrogel Microneedle Arrays with an Interpenetrating Bioelectroenzymatic Sensor for

Bastien Darmau^1,2, Marta Sacchi², Isabelle Texier²

¹Department of Molecular Chemistry, Univ. Grenoble Alpes-CNRS, 38041 Grenoble, France.

Advanced Healthcare Materials

|November 24, 2024

Summary

This study introduces novel hydrogel microneedle sensors for continuous diabetes monitoring. These microneedles offer a comfortable, stable, and simple alternative for long-term biomarker tracking.

Keywords:

biopolymers continuous glucose monitoring electrochemical sensors in vivo bioelectrocatalysis wearable biosensors

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Published on: November 28, 2017

Area of Science:

Biomedical Engineering
Materials Science
Analytical Chemistry

Background:

Continuous glucose monitors (CGMs) are vital for diabetes management but face challenges in cost, invasiveness, and stability.
Existing microneedle (MN) arrays often suffer from complex fabrication, poor mechanical/sensor stability, and cytotoxicity.

Purpose of the Study:

To develop and characterize novel crosslinked hydrogel microneedle-bioelectroenzymatic sensor arrays for continuous transdermal biomarker monitoring.
To evaluate the fabrication simplicity, stability, and performance of these microneedle sensors for extended use.

Main Methods:

Micromolding of dextran-methacrylate (Dex-MA) followed by dry-state visible light crosslinking to fabricate hydrogel MN arrays.
Integration of prefabricated electrodes and evaluation of polymer concentration effects on sensor properties.
Assessment of cytotoxicity and transdermal monitoring capabilities in artificial interstitial fluid for 10 days.

Main Results:

Demonstrated a simple, reproducible fabrication process for robust Dex-MA microneedles with shape control.
Showcased stable, biocompatible microneedle sensors capable of continuous transdermal monitoring for 10 days.
Confirmed that polymer substitution and mass content influence mechanical, swelling, and bioelectrocatalytic properties.

Conclusions:

Developed the first crosslinked hydrogel microneedle-bioelectroenzymatic sensor arrays for continuous biomarker monitoring.
The polysaccharide-based microneedle technology offers a promising, less invasive alternative for long-term health monitoring.
The integrated biosensors exhibited excellent cytotoxicity protection and flux-limiting capabilities.