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Updated: Jun 12, 2025
Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets
Published on: March 27, 2014
Protocol for reticulocyte enrichment from low-volume human blood samples from alpha-thalassemic and healthy
Stefano Comità1, Paola Valentino1, Teresa Ceglie1
1Department of Clinical and Biological Sciences, University of Turin, via Verdi 8, 10124, Turin, Italy.
Reticulocyte isolation from peripheral blood is crucial for hematological research. Here, we present a protocol for high-quality reticulocyte enrichment from small blood quantities obtained from alpha-thalassemic and healthy participants. We describe steps for Ficoll and Percoll gradient centrifugation to obtain a reticulocyte-enriched fraction, followed by negative immunomagnetic separation to remove granulocytes and platelets. This technique allows enriched reticulocyte isolation for multi-omics hematological analysis. Additionally, we detail procedures to recover peripheral blood mononuclear cells (PBMCs) and erythrocytes from the original blood sample.
Reticulocyte isolation from peripheral blood is crucial for hematological research. Here, we present a protocol for high-quality reticulocyte enrichment from small blood quantities obtained from alpha-thalassemic and healthy participants. We describe steps for Ficoll and Percoll gradient centrifugation to obtain a reticulocyte-enriched fraction, followed by negative immunomagnetic separation to remove granulocytes and platelets. This technique allows enriched reticulocyte isolation for multi-omics hematological analysis. Additionally, we detail procedures to recover peripheral blood mononuclear cells (PBMCs) and erythrocytes from the original blood sample.
