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Updated: Jun 6, 2025
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
MiR-185-5p is Involved in Regulating the Abnormal Proliferation of Retinal Microvascular Endothelial Cells via
Xiaoxia Wen1, Yunxia Tang2, Hongjian Guo2
1Department of Ophthalmology, The People's Hospital of Kaizhou District, Chongqing, China.
Purpose:
This study aimed to explore the expression profile of miR-185-5p in proliferative DR (PDR), and further evaluate its diagnostic value and possible mechanism of miR-185-5p in PDR.
Methods:
The level of miR-185-5p was detected by qRT-PCR. The ROC curve was established to estimate the diagnostic ability of miR-185-5p. Transwell experiment and cell counting kit-8 (CCK-8) assays were conducted to assess the effect of miR-185-5p on the migration and proliferation of human retinal endothelial cells (HRECs) induced by high glucose. Enzyme linked immunosorbent assay (ELISA) was used to detect the concentrations of inflammatory factors. The luciferase reporter gene experiment was used to prove the interaction between miR-185-5p and CXCR4.
Results:
Compared to the control group, the expression of miR-185-5p was significantly up-regulated in both the type 2 diabetes mellitus (T2DM) group and the PDR groups, with higher levels in the PDR group than in the T2DM group. The ROC curve reveals that serum miR-185-5p can distinguish PDR patients from T2DM patients. MiR-185-5p levels in HRECs increased significantly after high glucose induction. High glucose induction also promoted the migration, proliferation and inflammation response of HRECs. However, when the intracellular miR-185-5p level was down-regulated by miR-185-5p inhibitor transfection, these effects were inhibited. The luciferase reporter gene assay showed that miR-185-5p directly targets CXCR4.
Conclusion:
The expression of miR-185-5p is out of balance in PDR and it may be involved in regulating the migration and proliferation of HRECs by regulating CXCR4.
Insights
MicroRNA-185-5p is elevated in proliferative diabetic retinopathy (PDR) and may help diagnose PDR. It also influences retinal endothelial cell behavior by targeting CXCR4.
Area of Science:
- Ophthalmology
- Molecular Biology
- Biochemistry
Background:
- Diabetic retinopathy (DR) is a leading cause of vision loss.
- Proliferative diabetic retinopathy (PDR) is an advanced stage characterized by neovascularization.
- Understanding the molecular mechanisms underlying PDR is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the expression profile of miR-185-5p in PDR.
- To evaluate the diagnostic potential of miR-185-5p for PDR.
- To elucidate the role and mechanism of miR-185-5p in PDR pathogenesis.
Main Methods:
- Quantitative reverse transcription polymerase chain reaction (qRT-PCR) for miR-185-5p expression.
- Receiver operating characteristic (ROC) curve analysis for diagnostic evaluation.
- Transwell and CCK-8 assays to assess cell migration and proliferation.
- Enzyme-linked immunosorbent assay (ELISA) for inflammatory factors.
- Luciferase reporter gene assay to confirm target interaction.
Main Results:
- miR-185-5p was significantly upregulated in type 2 diabetes mellitus (T2DM) and PDR patients, with higher levels in PDR.
- Serum miR-185-5p demonstrated diagnostic capability to distinguish PDR from T2DM.
- High glucose increased miR-185-5p expression, promoting HREC migration, proliferation, and inflammation.
- Inhibition of miR-185-5p reversed these effects.
- miR-185-5p directly targets CXCR4.
Conclusions:
- miR-185-5p expression is dysregulated in PDR.
- miR-185-5p may play a role in PDR development by regulating HREC migration and proliferation via CXCR4.
