Artificial Insemination as a Possible Convenient Tool to Acquire Genome-Edited Mice via In Vivo Fertilization with...

Area of Science:

• Animal biotechnology
• Reproductive technologies
• Genetics and genomics

Background:

• Genome editing technologies enable rapid creation of genetically engineered (GE) animals for research and disease modeling.
• Current methods for producing GE animals primarily involve manipulating pre-implantation embryos in vitro.
• Producing GE animals via fertilization with genetically engineered sperm remains challenging, with limited successful reports.

Purpose of the Study:

• To review artificial insemination (AI) methods for producing GE animals.
• To discuss the history and potential of sperm-mediated gene transfer.
• To explore the feasibility of AI-mediated creation of GE animals using GE sperm.

Main Methods:

• Review of existing literature on artificial insemination techniques.
• Analysis of sperm-mediated gene transfer methodologies.
• Evaluation of in vivo fertilization approaches using genetically modified sperm.

Main Results:

• Artificial insemination offers a simpler alternative to in vitro fertilization for producing offspring.
• Sperm-mediated gene transfer has a history as a method for producing GE animals.
• The potential exists for AI-mediated creation of GE animals using GE sperm.

Conclusions:

• Artificial insemination presents a more efficient and less labor-intensive approach for generating genetically engineered animals.
• Further research into sperm-mediated gene transfer and AI techniques could advance the production of GE animal models.
• Genetically engineered sperm combined with AI holds promise for simplifying the creation of GE animals.