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Updated: Jun 6, 2025

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
Monoclonal Antibody Production Against Filoviruses from Immunized Mice
Guodong Liu1, Logan Banadyga2,3
1Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada. guodong.liu@phac-aspc.gc.ca.
Abstract:
Currently, targeted therapeutics against pathogenic filoviruses are very limited, with only two monoclonal antibody (mAb)-based products targeting Ebola virus having received approval from health authorities. More therapeutics are needed to fight against other fatal filoviruses. Here, we describe a protocol using hybridoma technology to generate mAbs that can target not only a single filovirus species but also multiple species for broad protection.

