Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

11.1K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
11.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

Nucleic acids research·2026
Same author

Versatile Sulfonium Tools for Chemoselective Modification of Proteins.

Biochemistry·2026
Same author

A proteomic atlas of organelle remodeling identifies lysosomal SNX3 as a regulator of Notch signaling in epidermal differentiation.

bioRxiv : the preprint server for biology·2026
Same author

Mutational Profiling Links Biologically Relevant Variants to Multi-Systemic Rosai-Dorfman Disease.

Blood advances·2026
Same author

Precise ^{136}Xe Double Beta Decay Measurement in PandaX-4T with Implications on the Nuclear Matrix Elements and Majorons.

Physical review letters·2026
Same author

Interaction of NDRG1 and MRE11 Modulates DNA Replication and Repair.

Cancers·2026
Same journal

A Ni-Mediated Cross-Coupling Approach to Deuterated <sup>18</sup>F- Fluoromethylated (Hetero)arenes.

Journal of the American Chemical Society·2026
Same journal

Efficient Light-Driven CO<sub>2</sub> Capture and Reversible Release Enabled by Metastable Photoacid-Decorated Metal-Organic Frameworks.

Journal of the American Chemical Society·2026
Same journal

In Situ Raman Spectroscopy Reveals the Dynamic Evolution and Ethanol Dependence of SEI Structure in Li-Mediated N<sub>2</sub> Reduction Reaction.

Journal of the American Chemical Society·2026
Same journal

Solvent Esterification and Stoichiometric Control in Ambient-Grown FAPbI<sub>3</sub> Single-Crystal Solar Cells.

Journal of the American Chemical Society·2026
Same journal

Unlocking Azulene Functionalization via Strain-Induced Azulyne Intermediates.

Journal of the American Chemical Society·2026
Same journal

An Oxazine-Locked Covalent Organic Framework by a Tandem Pinner/Schiff Base Reaction for Hydrogen Peroxide Photosynthesis.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: Jun 6, 2025

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

18.2K

Circular Engineered Sortase for Interrogating Histone H3 in Chromatin.

Samuel D Whedon1, Kwangwoon Lee1, Zhipeng A Wang1

  • 1Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, United States.

Journal of the American Chemical Society
|November 25, 2024
PubMed
Summary
This summary is machine-generated.

A new enzyme, cW11 sortase, enables precise modification of histone H3 tails for studying epigenetic regulation and developing new therapies. This method accelerates the creation of modified nucleosomes and aids in analyzing histone modification crosstalk.

More Related Videos

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
05:58

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

1.0K
Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
07:26

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

Published on: December 26, 2020

4.0K

Related Experiment Videos

Last Updated: Jun 6, 2025

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

18.2K
Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
05:58

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

1.0K
Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
07:26

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

Published on: December 26, 2020

4.0K

Area of Science:

  • Epigenetics and Molecular Biology
  • Chromatin Biology
  • Proteomics

Background:

  • Histone H3 N-terminal tail modifications regulate chromatin structure, gene expression, and cell states.
  • Dysregulation of these modifications is linked to disease pathogenesis.
  • Understanding crosstalk between H3 tail modifications is a key challenge in epigenetics.

Purpose of the Study:

  • To develop a method for scarless introduction of H3 tails onto nucleosomes.
  • To facilitate the production of symmetrically and asymmetrically modified nucleosomes.
  • To enable quantitative analysis of histone modification crosstalk and its impact on molecular recognition and enzyme processing.

Main Methods:

  • Engineered sortase transpeptidase (cW11) for scarless H3 tail modification.
  • Chemoenzymatic synthesis of symmetrically and asymmetrically modified nucleosomes.
  • Multiplex "cut-and-paste" middle-down proteomics using tandem mass tags.

Main Results:

  • cW11 sortase efficiently produces scarless H3 tails on nucleosomes, accelerating modified nucleosome production.
  • Asymmetrically modified nucleosomes were used to dissect the impact of multiple modifications on enzyme processing and protein recognition.
  • cW11 facilitated quantitative analysis of histone H3 modification crosstalk using a novel proteomics approach.

Conclusions:

  • The cW11 sortase is a powerful tool for epigenetic research, enabling efficient and precise modification of histone H3 tails.
  • This chemoenzymatic strategy significantly advances the study of histone modification crosstalk.
  • The developed methods hold promise for future epigenetic discovery and therapeutic development.