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Updated: Jun 10, 2026

Glycan Node Analysis: A Bottom-up Approach to Glycomics

Glycan Node Analysis: A Bottom-up Approach to Glycomics

Published on: May 22, 2016

Exploring Glycan-Lectin Interactions in Natural-Like Environments: A View Using NMR Experiments Inside Cell and on

Sara Bertuzzi¹, Marta G Lete¹, Antonio Franconetti¹

¹Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160, Derio, Bizkaia, Spain.

Chemistry (Weinheim an Der Bergstrasse, Germany)

|November 26, 2024

Summary

Nuclear Magnetic Resonance (NMR) methods were adapted to study glycan-lectin interactions within cellular environments. This research demonstrates the feasibility of observing these crucial biological recognition events in native-like settings.

Keywords:

glycans in-cell NMR lectins molecular recognition on-cell NMR

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Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition

Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition

Published on: May 17, 2024

Area of Science:

Biochemistry
Molecular Biology
Biophysics

Background:

Glycan-mediated molecular recognition is vital for cellular processes.
Nuclear Magnetic Resonance (NMR) is a key technique for studying glycan-lectin interactions in vitro.
Existing NMR methods often use isolated components, not mimicking native cellular conditions.

Purpose of the Study:

To develop and apply NMR methodologies for monitoring glycan-lectin interactions within cellular environments.
To investigate glycan recognition from both receptor and ligand perspectives under native-like conditions.
To establish proof-of-concept for NMR analysis of glycan recognition in situ.

Main Methods:

Exploration of diverse NMR techniques, including 1H-15N HMQC NMR for intracellular observations and ligand-based STD-NMR for cell surface interactions.
Utilized Danio Rerio oocytes for in-cell NMR experiments with galectin-7 and a glycomimetic ligand (TDG).
Applied STD-NMR to study interactions of natural glycans and glycomimetics with cell surface-expressed Siglec-10.

Main Results:

Successfully observed the 1H-15N HMQC NMR spectrum of folded galectin-7 inside Danio Rerio oocytes, a first for in-cell NMR of galectins.
Demonstrated the use of a glycomimetic ligand (TDG) to facilitate intracellular galectin-7 NMR studies.
Successfully applied STD-NMR to characterize glycan-Siglec-10 interactions on the cell surface.

Conclusions:

NMR methodologies can be adapted to study glycan-lectin interactions in native-like cellular environments, both intracellularly and on the cell surface.
These advanced NMR approaches provide new tools for understanding the biological roles of glycan recognition in situ.
The study opens avenues for future NMR investigations into complex biological recognition events within their native cellular context.