Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same authorSame journal

A Review: The Progress of Oxadiazole Derivatives in Agrochemical Application.

Journal of agricultural and food chemistry·2026

Same author

Multimodal assessment of balance dysfunction in older adults: from classification to clustering-based functional pattern identification.

Journal of neuroengineering and rehabilitation·2026

Same author

Research progress and trends on DNA methylation research in non-small cell lung cancer: a bibliometric analysis.

Journal of thoracic disease·2026

Same author

Design, Synthesis, and Insecticidal Potential of 1,2,4-Oxadiazole-Fused Isoxazoline Derivatives as GABA Receptor Antagonists.

Journal of agricultural and food chemistry·2026

Same author

ChroB Site on the GABA RDL Receptor as a Potential Insecticide Target.

Journal of agricultural and food chemistry·2026

Same author

Age-related interplay of walking economy, stability, and neuromuscular coordination during single- and dual-task walking.

Experimental gerontology·2026

Search research articles

Related Experiment Video

Updated: Jun 6, 2025

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae

Published on: August 5, 2022

Fluorescent Probes Visualize Phytohormone: Research Status and Opportunities.

Yaming Liu¹, Anjing Liao¹, Shunhong Chen¹

¹State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, China.

Journal of Agricultural and Food Chemistry

|November 26, 2024

Summary

Fluorescent probes enable visualization of plant hormones, aiding crop stress resistance. Further research is needed to overcome challenges in high-resolution imaging and biocompatibility for advanced plant growth regulation.

Keywords:

distribution fluorescent probe phytohormone quantum dot visualization

More Related Videos

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor

Published on: January 12, 2019

Related Experiment Videos

Last Updated: Jun 6, 2025

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae

Published on: August 5, 2022

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor

Published on: January 12, 2019

Area of Science:

Plant Science
Biochemistry
Analytical Chemistry

Background:

Phytohormones regulate plant growth and stress responses.
Understanding phytohormone distribution is key for crop improvement and plant growth regulator development.
Fluorescent probe technology offers a promising method for monitoring phytohormones.

Purpose of the Study:

To highlight the utility of fluorescent probes for measuring and visualizing phytohormone distribution in plants under stress.
To discuss the challenges and opportunities in applying fluorescent probes for phytohormone analysis.
To provide insights for developing and using plant growth regulators.

Main Methods:

Utilizing fluorescent probe technology for phytohormone detection.
Employing advanced imaging methods for visualization.
Analyzing probe response to phytohormones in plant systems.

Main Results:

Fluorescent probes are effective in detecting phytohormone responses in plants.
The technique allows for visualization of phytohormone distribution under external stress.
Challenges remain in achieving high spatial resolution and biocompatibility.

Conclusions:

Fluorescent probes are valuable tools for studying plant hormone dynamics.
Further development is needed to address current limitations in imaging and biocompatibility.
This technology holds potential for enhancing crop stress resistance and guiding plant growth regulator applications.