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Updated: Jun 6, 2025

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Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR
Published on: February 4, 2018
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Impact of Revised Diagnostic Testing Algorithms and Case Definitions on Lyme Disease Surveillance in a Low-Incidence
Gayoung Lee1, Brandon D Hollingsworth2,3, Dana A Giandomenico4
1Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Abstract:
We evaluated the impact of the endorsement of modified 2-tiered testing and its inclusion in updated Lyme disease case definitions in North Carolina using surveillance data from 2017-2023. After the implementation of modified 2-tiered testing, confirmed cases increased by 117%, with comparable decreases in probable cases compared with prior periods.

