Vancomycin-Loaded Silk Fibroin/Calcium Phosphate/Methylcellulose-Based In Situ Thermosensitive Hydrogel: A Potential
Premchirakorn Phewchan1,2, Artit Laoruengthana3, Pratthana Chomchalao4
1Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand.
Gels (Basel, Switzerland)
|November 26, 2024
Summary
This study shows vancomycin-loaded hydrogels promote bone cell growth and differentiation. The formulation with high silk fibroin and calcium phosphate content is most effective for bone regeneration.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Osteomyelitis and bone defects require effective treatments.
- Silk fibroin/calcium phosphate/methylcellulose hydrogels offer potential for bone regeneration.
Purpose of the Study:
- To evaluate vancomycin-loaded hydrogels for osteogenic differentiation.
- To compare formulations with varying silk fibroin and calcium phosphate concentrations.
Main Methods:
- Prepared three vancomycin-loaded silk fibroin/calcium phosphate/methylcellulose hydrogels (VM-SF/CaP/MC).
- Assessed MC3T3-E1 cell migration, proliferation, alkaline phosphatase (ALP) activity, collagen content, and mineralization.
- Compared the efficacy of VM-HSF/LCaP/MC, VM-LSF/HCaP/MC, and VM-HSF/HCaP/MC formulations.
Main Results:
- All hydrogels enhanced MC3T3-E1 cell migration and proliferation.
- Significant promotion of ALP activity, collagen content, and mineralization was observed.
- The VM-HSF/HCaP/MC hydrogel showed superior osteogenic potential, with the highest ALP activity, collagen, and mineralization.
Conclusions:
- Vancomycin-loaded silk fibroin/calcium phosphate/methylcellulose hydrogels effectively promote osteogenic differentiation.
- The VM-HSF/HCaP/MC formulation is a promising candidate for clinical bone regeneration, especially for osteomyelitis.
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