Extraction Optimization of Polysaccharides from Wet Red Microalga Porphyridium purpureum Using Response Surface...

Area of Science:

• Marine Biology
• Biotechnology
• Biochemistry

Background:

• Porphyridium is a marine microalga rich in bioactive polysaccharides.
• Optimizing polysaccharide extraction enhances the value of Porphyridium biomass.
• Polysaccharides from microalgae have significant biological activities.

Purpose of the Study:

• To optimize polysaccharide extraction conditions from Porphyridium using response surface methodology (RSM).
• To evaluate the effectiveness of microwave-assisted extraction for improving polysaccharide yield.
• To determine the optimal parameters for maximizing polysaccharide recovery.

Main Methods:

• Response surface methodology (RSM) was employed to optimize extraction time, temperature, and biomass-to-water ratio.
• Microwave-assisted extraction was utilized to further enhance polysaccharide yield.
• Multiple regression analysis was performed to analyze the RSM results.

Main Results:

• Increased extraction temperature and time positively affected polysaccharide yield.
• Optimal conditions determined were 45 min extraction time, 87 °C temperature, and a 1:63 g mL-1 biomass-to-water ratio.
• Maximum polysaccharide yield achieved was 23.66% DW under optimal conditions, with microwave-assisted extraction increasing it to 25.48% DW.
• Extraction temperature was identified as the most critical factor influencing yield.

Conclusions:

• Hot water extraction combined with microwave assistance is an effective method for Porphyridium purpureum polysaccharide extraction.
• The developed RSM model accurately predicts and optimizes polysaccharide yield.
• Optimized extraction significantly increases the recovery of valuable polysaccharides from microalgal biomass.