Detection and quantification of lactoferrin: Innovations, applications, and challenges
Reza Abedi-Firoozjah1, Behnam Behnam2, Zakiyeh Balouch Zehi2
1Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Food Chemistry
|November 26, 2024
Summary
This review explores advanced analytical methods for detecting lactoferrin (LF), an 80 kDa glycoprotein with vital immune functions. It highlights new biosensor developments using nanomaterials for improved sensitivity and performance in food safety applications.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Food Science
Background:
- Lactoferrin (LF) is an 80 kDa iron-binding glycoprotein with diverse biological functions, including host defense, anti-inflammatory, antioxidant, antimicrobial, and antiparasitic activities.
- Accurate analytical methods are essential for ensuring food safety, quality control, and implementing hazard analysis and good manufacturing practices in food production.
Purpose of the Study:
- To review recent advancements in analytical methods for lactoferrin (LF) detection.
- To provide an in-depth analysis of the applications, advantages, and disadvantages of conventional and modern analytical techniques for LF detection.
- To explore the latest developments in biosensors for LF detection, focusing on nanomaterial applications and performance evaluation.
Main Methods:
- Review of existing literature on analytical techniques for lactoferrin detection.
- Analysis of conventional and modern analytical methods, including their strengths and weaknesses.
- Exploration of biosensor development, including nanomaterial-based signal probes and carriers, sensitivity, and performance metrics.
Main Results:
- Identification of various analytical techniques for lactoferrin detection, ranging from conventional to modern approaches.
- Detailed comparison of the advantages and limitations of different analytical methods.
- Overview of emerging biosensor technologies utilizing nanomaterials for enhanced sensitivity and efficiency in lactoferrin detection.
Conclusions:
- Advancements in analytical methods, particularly biosensors, offer improved sensitivity and performance for lactoferrin detection.
- Nanomaterials play a crucial role in enhancing biosensor capabilities for LF detection.
- Further research into biosensor development holds significant potential for optimizing lactoferrin analysis in various applications, especially food safety.
