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Related Experiment Video

Updated: Jun 6, 2025

Assessment of the Metabolic Profile of Primary Leukemia Cells

Published on: November 21, 2018

Decoding Acute Myeloid Leukemia: A Clinician's Guide to Functional Profiling.

Prasad Iyer^1,2, Shaista Shabbir Jasdanwala³, Yuhan Wang³

¹Children's Blood and Cancer Centre, KK Women's and Children's Hospital, Singapore 229899, Singapore.

Diagnostics (Basel, Switzerland)

|November 27, 2024

Summary

Functional profiling techniques offer new insights into acute myeloid leukemia (AML) biology. These methods identify vulnerabilities and potential therapeutic targets, improving AML patient outcomes.

Keywords:

BH3 profiling acute myeloid leukemia drug sensitivity/resistance testing functional profiling gene expression profiling metabolomics proteomics

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Area of Science:

Hematology
Molecular Biology
Cancer Research

Background:

Acute myeloid leukemia (AML) is a heterogeneous clonal disorder with significant therapeutic challenges.
Despite advances, understanding AML's genetic, metabolic, and epigenetic landscape remains complex.
Aberrant proliferation of precursor cells characterizes AML, necessitating novel diagnostic and therapeutic strategies.

Purpose of the Study:

To review diverse functional profiling techniques for understanding acute myeloid leukemia (AML) biology.
To highlight the advantages, limitations, and successes of these methods in AML research.
To explore the potential of functional profiling in guiding personalized AML treatment and improving patient outcomes.

Main Methods:

BH3 profiling (BP) assesses mitochondrial apoptotic thresholds.
Gene expression profiling (GEP) analyzes transcriptomic alterations.
Proteomics, metabolomics, drug sensitivity/resistance testing (DSRT), and CRISPR/RNAi screens offer functional insights.
Multi-omics approaches and artificial intelligence integration are advancing precision.

Main Results:

Functional profiling identifies AML-specific vulnerabilities and biomarkers.
Techniques reveal drug resistance mechanisms and potential drug repurposing opportunities.
Studies have linked specific mutations and gene signatures to aggressive AML subtypes.

Conclusions:

Functional profiling methods provide critical insights into AML heterogeneity and behavior.
These techniques are instrumental in identifying novel therapeutic targets and predicting treatment response.
Integrating functional profiling into routine AML care holds promise for improved patient outcomes.