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Updated: Jun 6, 2025
Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique
Published on: September 23, 2022
Comparison οf Immune Responses Through Multiparametric T-Cell Cytokine Expression Profile Between Children with
Filippos Filippatos1, Marianna Tzanoudaki2, Elizabeth-Barbara Tatsi3
1Infectious Diseases and Chemotherapy Research Laboratory, First Department of Pediatrics, Medical School, National and Kapodistrian University of Athens, "Aghia Sophia" Children's Hospital, 11527 Athens, Greece.
Background/Objectives:
The immunological pathways that cause Multisystem Inflammatory Syndrome after SARS-CoV-2 infection in children (MIS-C) remain under investigation.
Methods:
The aim of this study was to prospectively compare the T-cell cytokine expression profile in unvaccinated children with acute MIS-C (MISC_A) before immunosuppression, convalescent MIS-C (one month after syndrome onset, MISC_C), convalescent COVID-19 (one month after hospitalization), and in healthy, unvaccinated controls. The intracellular expression of IL-4, IL-2, IL-17, IFNγ, TNF-α and Granzyme B, and the post SARS-CoV-2-Spike antigenic mix stimulation of T-cell subsets was analyzed by 13-color flow cytometry.
Results:
Twenty children with a median age (IQR) of 11.5 (7.25-14) years were included in the study. From the comparison of the flow cytometry analysis of the 14 markers of MISC_A with the other three groups (MISC_C, post-COVID-19 and controls), significant differences were identified as follows: 1. CD4+IL-17+/million CD3+: 293.0(256.4-870.9) vs. 50.7(8.4-140.5); p-value: 0.03, vs. 96.7(89.2-135.4); p-value: 0.03 and vs. 8.7(0.0-82.4); p-value: 0.03, respectively; 2. CD8+IL-17+/million CD3+: 335.2(225.8-429.9) vs. 78.0(31.9-128.9) vs. 84.1(0.0-204.6) vs. 33.2(0.0-114.6); p-value: 0.05, respectively; 3. CD8+IFNγ+/million CD3+: 162.2(91.6-273.4) vs. 41.5(0.0-77.4); p-value: 0.03 vs. 30.3(0.0-92.8); p-value: 0.08, respectively.
Conclusions:
In children presenting with MIS-C one month after COVID-19 infection, T cells were found to be polarized towards IL-17 and IFNγ production compared to those with uncomplicated convalescent COVID-19, a finding that could provide possible immunological biomarkers for MIS-C detection.
