Assessing Antiparasitic Compounds Persistence in Cattle Hair by DART-MS

Area of Science:

• Analytical Chemistry
• Veterinary Toxicology
• Mass Spectrometry

Background:

• Antiparasitic agents are crucial for cattle health and productivity.
• Accurate monitoring of antiparasitic drug persistence in animal matrices is essential for efficacy and safety.
• Existing analytical methods can be time-consuming or require extensive sample preparation.

Purpose of the Study:

• To develop and validate a Direct Analysis in Real Time Mass Spectrometry (DART-MS) method for quantifying specific antiparasitic compounds in cattle hair.
• To evaluate the analytical performance of the DART-MS method for precision, accuracy, and limits of detection.
• To establish a rapid and efficient analytical strategy for assessing antiparasitic persistence in cattle.

Main Methods:

• Direct Analysis in Real Time Mass Spectrometry (DART-MS) was employed for sample analysis.
• Positive ion mode was utilized for DART-MS analyses.
• Key parameters of the DART source were optimized to enhance analytical performance.
• Quantification of fenthion, chlorpyrifos, and cypermethrin in cattle hair samples was performed.

Main Results:

• The optimized DART-MS method demonstrated high efficiency for the quantification of fenthion, chlorpyrifos, and cypermethrin.
• The method met established criteria for precision, accuracy, and limits of detection.
• Analytical performance confirmed the suitability of DART-MS for routine analysis of these antiparasitic agents.

Conclusions:

• The developed DART-MS method offers a fast and efficient approach for determining antiparasitic agents in cattle hair.
• This analytical strategy supports the evaluation of drug administration protocols and dosage intervals.
• The method contributes to enhancing the safety and advancement of the livestock sector through reliable residue analysis.