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Related Concept Videos

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Related Experiment Video

Updated: Jun 19, 2026

A Fluorescent Screening Assay for Identifying Modulators of GIRK Channels
05:31

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Published on: April 24, 2012

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High-Performance Chemigenetic Potassium Ion Indicator.

Dazhou Cheng1, Zhenlin Ouyang2, Xiaoyu He3

  • 1Department of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.

Journal of the American Chemical Society
|November 27, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed new red-fluorescent indicators for potassium ions (K+) called HaloKbp1. These genetically targetable sensors offer high specificity and brightness, enabling detection of cellular K+ changes, such as those from BK channel activity.

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Area of Science:

  • Biochemistry and Molecular Biology
  • Cellular Physiology
  • Fluorescent Probes and Imaging

Background:

  • Potassium ion (K+) is crucial for cellular functions, making accurate measurement vital.
  • Existing fluorescent K+ indicators lack genetic targetability, high specificity, or red/far-red emission.
  • A need exists for advanced K+ sensors with improved performance characteristics for biological research.

Purpose of the Study:

  • To develop novel chemigenetic fluorescent indicators for intracellular potassium ion (K+) detection.
  • To create indicators with high specificity for K+ over sodium ions (Na+).
  • To achieve indicators with high brightness and large fluorescence changes in the red to far-red spectrum.

Main Methods:

  • Engineered a series of chemigenetic K+ indicators (HaloKbp1) using a bacterial K+-binding protein (Kbp) fused to HaloTag7.
  • Incorporated environmentally sensitive rhodamine derivatives for fluorescence signaling.
  • Validated indicator performance in detecting physiologically relevant K+ concentration changes.

Main Results:

  • The HaloKbp1 series exhibits high brightness in the red to far-red wavelength range.
  • These indicators demonstrate large intensiometric fluorescence changes and tunable dissociation constants (Kd).
  • Successfully detected K+ concentration changes linked to Ca2+-activated BK channel activity.

Conclusions:

  • The HaloKbp1 series represents a significant advancement in K+ indicator technology.
  • These indicators are suitable for genetically targeting and visualizing K+ dynamics in biological systems.
  • The developed sensors provide a valuable tool for studying cellular electrophysiology and signaling pathways.