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Using Hybrid Coating to Fabricate Highly Stable and Expandable Transparent Liquid Marbles.

Renis Agosto Nieves1, Gabriela Gomez Dopazo1,2, Joseph Rosenfeld2

  • 1Department of Chemistry, University of Puerto Rico at Cayey, 205 Ave. Antonio R Barcelo, Cayey, Puerto Rico 00736, United States.

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Summary

This study developed advanced liquid marbles (LMs) using a hybrid coating of nano- and microparticles for sensitive colorimetric assays. These versatile microreactors offer a portable, instrument-free diagnostic tool with high accuracy and precision.

Keywords:
coalescencecolorimetric methodsevaporation timehybrid coatinghydrophobic coatingsmicroreactorsilanized fumed silicasilanized silica gel

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Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Liquid marbles (LMs) are droplet-based microreactors with properties influenced by particle coatings.
  • Existing LM coatings have limitations in transparency, expandability, and stability for certain applications.
  • Developing robust LMs is crucial for advancing microfluidic and diagnostic technologies.

Purpose of the Study:

  • To fabricate and characterize liquid marbles (LMs) using a hybrid coating of modified silica gel (MSG) and modified fumed silica (MFS) particles.
  • To evaluate the performance of these hybrid LMs (MIX LMs) in colorimetric assays compared to LMs with single-component coatings.
  • To demonstrate the potential of MIX LMs as versatile microreactors for sensitive and portable diagnostic applications.

Main Methods:

  • Fabrication of LMs using a hybrid coating mixture (MIX) of MSG (40-75 μm) and MFS (200-300 nm) in a 3:7 (w/w) ratio.
  • Characterization of LM properties including transparency, expandability, evaporation resistance, and coalescence resistance.
  • Performance evaluation of MIX LMs in three different colorimetric assays (alkaline phosphatase, bovine serum albumin, chymotrypsin) and quantification using a smartphone application.

Main Results:

  • MIX LMs exhibited high transparency, over 20-fold expandability, and stability against evaporation for over 4 hours.
  • The hybrid coating significantly improved resistance to coalescence, attributed to the presence of different-sized particles.
  • Colorimetric assays in MIX LMs yielded results comparable in accuracy and precision to standard microwell plates, with low limits of detection and quantification.

Conclusions:

  • Hybrid coatings of nano- and microparticles create robust and versatile liquid marbles suitable for microreactor applications.
  • MIX LMs offer a promising platform for sensitive, portable, and instrument-free colorimetric assays.
  • This technology paves the way for more accessible and efficient diagnostic tools.