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Related Concept Videos

CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Engineering stimuli-responsive CRISPR-Cas systems for versatile biosensing.

Linxin Cao1, Wenhui Chen1, Wenyuan Kang2

  • 1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha, 410082, Hunan, China.

Analytical and Bioanalytical Chemistry
|November 27, 2024
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Summary
This summary is machine-generated.

Engineered CRISPR-Cas systems offer precise target recognition for biosensing. These systems can be modified to detect various non-nucleic acid analytes, enhancing diagnostic capabilities.

Keywords:
BiosensingCRISPR-CasFunctional nucleic acidProtein engineering

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Area of Science:

  • Biotechnology and Biosensing
  • Molecular Biology
  • Bioengineering

Background:

  • CRISPR-Cas systems are recognized for their precise target recognition and signal amplification in biosensing.
  • Existing CRISPR-Cas systems are being engineered for stimulus-responsive functionalities.
  • Protein and nucleic acid engineering techniques are employed to create conditional CRISPR-Cas systems.

Purpose of the Study:

  • To review recent advancements in engineering CRISPR-Cas systems for stimulus-responsive biosensing.
  • To summarize the development of conditional CRISPR-Cas systems for detecting non-nucleic acid analytes.
  • To discuss the challenges and future prospects of stimulus-responsive CRISPR-Cas systems in biosensing.

Main Methods:

  • Engineering of Cas proteins to incorporate stimulus-responsive elements.
  • Modification of guide RNA (gRNA) to achieve target analyte-dependent activity.
  • Design of substrate nucleic acids with analyte-responsive features.

Main Results:

  • Development of diverse conditional CRISPR-Cas systems activated by specific target triggers.
  • Demonstration of efficient detection of various non-nucleic acid analytes using engineered CRISPR-Cas systems.
  • Establishment of analyte-responsive capabilities in key CRISPR-Cas biocomponents.

Conclusions:

  • Engineered CRISPR-Cas systems show significant potential for versatile and sensitive biosensing applications.
  • Stimulus-responsive CRISPR-Cas systems offer a promising platform for detecting a wide range of analytes.
  • Further research into challenges and possibilities will drive the future of this technology.