Jove
Visualize
Contact Us

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure of the pre-initiation complex explains CMGE biogenesis.

Nature·2026
Same author

Targeting Cancer-Specific Mutations with RNA-Triggered Chromatin Shredding.

Nature·2026
Same author

An ATP-gated molecular switch orchestrates human mRNA export.

Nature·2025
Same author

S-phase checkpoint protects from aberrant replication fork processing and degradation.

Nucleic acids research·2025
Same author

Cell cycle regulation has shaped replication origins in budding yeast.

Nature structural & molecular biology·2025
Same author

Mechanisms for licensing origins of DNA replication in eukaryotic cells.

Nature structural & molecular biology·2025
Same journal

Retraction Note: NSD2 targeting reverses plasticity and drug resistance in prostate cancer.

Nature·2026
Same journal

Enhanced B cell priming induces broadly neutralizing HIV-1 apex antibodies.

Nature·2026
Same journal

Vaccination elicits HIV broadly neutralizing antibodies in primates.

Nature·2026
Same journal

Child online safety needs more than social-media bans.

Nature·2026
Same journal

Ebola preparedness must start with ecosystems and before humans show symptoms.

Nature·2026
Same journal

AI tools can speed up thinking, but evidence still comes from the lab bench.

Nature·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 12, 2026

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples
07:40

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

Published on: May 5, 2017

11.3K

MCM double hexamer loading visualized with human proteins.

Florian Weissmann1, Julia F Greiwe2, Thomas Pühringer2

  • 1Chromosome Replication Laboratory, The Francis Crick Institute, London, UK.

Nature
|November 28, 2024
PubMed
Summary
This summary is machine-generated.

Human DNA replication initiation involves loading the MCM helicase. This study reveals distinct human double hexamer loading mechanisms compared to yeast, highlighting differences in protein interactions and assembly pathways.

More Related Videos

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
09:33

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

7.2K
Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

961

Related Experiment Videos

Last Updated: May 12, 2026

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples
07:40

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

Published on: May 5, 2017

11.3K
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
09:33

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

7.2K
Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

961

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Eukaryotic DNA replication initiates with MCM helicase loading as a double hexamer at replication origins.
  • Current models for double hexamer assembly by ORC, CDC6, and CDT1 are primarily based on budding yeast.

Purpose of the Study:

  • To biochemically reconstitute and structurally characterize human double hexamer (hDH) loading.
  • To elucidate the mechanistic differences in hDH assembly compared to the yeast double hexamer (yDH).

Main Methods:

  • Biochemical reconstitution using purified human proteins.
  • Cryo-electron microscopy (cryo-EM) for structural analysis.
  • Comparative analysis with existing yeast data.

Main Results:

  • The hDH engages DNA differently than yDH, creating underwound DNA at the hexamer interface.
  • Identified distinct factor recruitment orders and dependencies during hDH assembly.
  • ORC6 is not essential for initial MCM recruitment but facilitates an alternative pathway involving ORC1's disordered region.

Conclusions:

  • Human double hexamer assembly differs significantly from yeast, indicating diversity in eukaryotic replication initiation.
  • This work provides a detailed view of hDH assembly in an organism with sequence-independent origins.
  • Represents a foundational step towards reconstituting human DNA replication initiation in vitro.