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Genotype-by-environment interactions shape ubiquitin-proteasome system activity.

Randi R Avery1, Mahlon A Collins1, Frank W Albert1

  • 1Department of Genetics, Cell Biology, & Genetics, University of Minnesota, Minneapolis, MN, USA.

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|November 28, 2024
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Summary
This summary is machine-generated.

Genotype-by-environment interactions (GxE) significantly impact protein degradation, the cell's protein disposal system. This study reveals complex GxE in the ubiquitin-proteasome system (UPS) across different yeast environments.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Systems Biology

Background:

  • Genotype-by-environment interactions (GxE) are crucial for trait variation in eukaryotes.
  • Understanding GxE's role in molecular processes like protein degradation is limited.
  • Protein degradation via the ubiquitin-proteasome system (UPS) is vital for cellular function.

Purpose of the Study:

  • To investigate how GxE influences protein degradation.
  • To characterize GxE within the yeast ubiquitin-proteasome system (UPS).
  • To map genetic effects on UPS activity across diverse environments.

Main Methods:

  • Utilized the yeast *Saccharomyces cerevisiae* model system.
  • Mapped genetic influences on the degradation of six UPS substrates.
  • Analyzed UPS activity across eight distinct environmental conditions.

Main Results:

  • Discovered extensive GxE in the genetics of UPS activity.
  • Identified hundreds of genetic loci with environment- or substrate-dependent effects.
  • Observed both presence/absence and sign-change types of GxE.
  • Found GxE loci clustered near core UPS genes and expression regulatory regions.

Conclusions:

  • GxE plays a complex role in shaping protein degradation.
  • Genetic variation in UPS activity is highly sensitive to environmental context.
  • Indirect genetic effects significantly contribute to GxE in protein degradation.