Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A microphysiologic human cervical model recapitulates microbial, immune, and pathogenic properties of sexually transmitted infections.

Science advances·2026
Same author

BEADS: An Interactive Semi-Automated Workflow for 3D Fibrin Angiogenesis Assays Enabling Co-Culture and Directionality Analysis.

bioRxiv : the preprint server for biology·2025
Same author

Modular Parallel Plate Flow Chamber with Tunable Substrate Mechanics and Defined Shear Stress.

bioRxiv : the preprint server for biology·2025
Same author

Anatomical 3D Reconstruction of Murine Lymph Nodes for Visualization, Quantitation, and Numerical Simulation.

bioRxiv : the preprint server for biology·2025
Same author

Confocal-Compatible Workflow for Sectioning, Staining, and Imaging Serial Vibratome Sections for 3D Anatomical Reconstruction of the Lymph Node.

bioRxiv : the preprint server for biology·2025
Same author

Protein Language Models are Accidental Taxonomists.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Jun 6, 2025

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
10:17

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Published on: November 4, 2021

3.1K

Stochastic to Deterministic: A Straightforward Approach to Create Serially Perfusable Multiscale Capillary Beds.

Michael J Donzanti1, Bryan J Ferrick1, Omkar Mhatre1

  • 1Department of Biomedical Engineering, University of Delaware, Newark, Delaware United States 19713.

ACS Biomaterials Science & Engineering
|November 28, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel method to create perfusable, multiscale vascular networks in vitro. This technique enables direct investigation of blood vessel formation and remodeling in engineered tissues.

Keywords:
BiofabricationCellular Self-AssemblyIn Vitro Vascular ModelsMultiscale PatterningTissue EngineeringVasculogenesis

More Related Videos

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels
07:49

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Published on: January 14, 2021

3.4K
Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture
10:00

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Published on: July 20, 2022

2.1K

Related Experiment Videos

Last Updated: Jun 6, 2025

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
10:17

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Published on: November 4, 2021

3.1K
Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels
07:49

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Published on: January 14, 2021

3.4K
Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture
10:00

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Published on: July 20, 2022

2.1K

Area of Science:

  • Biomedical Engineering
  • Vascular Biology
  • Tissue Engineering

Background:

  • * Generating in vitro tissue models with hierarchical vasculature is crucial for studying blood vessel development and function.
  • * Existing methods often lack precise control over fluid perfusion and network architecture.

Purpose of the Study:

  • * To develop a method for producing closed, serially perfused, multiscale vascular networks within acellular hydrogels.
  • * To enable direct mechanistic investigation of vasculogenesis, angiogenesis, and vascular remodeling.
  • * To provide a low-barrier-to-entry technique for researchers in vascular biology.

Main Methods:

  • * Fabrication of a closed, multiscale vessel network embedded in an acellular hydrogel.
  • * Confirmation of functional annealing at the gel-gel interface.
  • * Validation of multiscale connectivity using high-resolution microscopy.
  • * Demonstration of perfusability using fluorescently labeled microspheres and directed flow.

Main Results:

  • * Successfully produced a closed, serially perfused, multiscale vascular network.
  • * Confirmed functional interface without hindering cell migration or endothelial self-assembly.
  • * Validated anastomosis between self-assembled and patterned vessels.
  • * Demonstrated directed flow through the capillary bed from inlet to outlet.

Conclusions:

  • * Developed a straightforward method for fabricating perfusable, multiscale vascular networks.
  • * The technique facilitates mechanistic studies in vascular biology.
  • * Offers potential for integration into organoid culture, organ-on-a-chip models, and bioprinted tissues.