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Updated: Jun 6, 2025
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
What can your microbiota tell you about your health?
Ozgenur Hacioglu1, Zeynep Ozoral1
1Kirklareli University, Faculty of Health Sciences, Department of Midwifery - Kırklareli, Turkey.
Objective:
As healthy microbiota lays the foundation, it is crucial for midwives, who play an important role and responsibility in providing necessary recommendations for strengthening the connection between the mother and the child, to understand the basic structures and functions of various microbial communities and to be knowledgeable about microbiota and health. Therefore, this study aimed to provide microbiota education to midwifery students to explain the importance of microbiota for health, demonstrate its relationship with newborn health, women's health, and diseases, and evaluate the students' awareness of microbiota at the end of the education.
Methods:
The education provided within the scope of the research was conducted face-to-face over 4 weeks, with 2 h of education per week, totaling 8 h. The students' level of knowledge about microbiota was assessed before and 3 months after the education using the microbiota knowledge test, and statistical analysis was conducted.
Results:
The education provided within the scope of the research was conducted face-to-face over 4 weeks, with 2 h of education per week, totaling 8 h. The students' level of knowledge about microbiota was assessed before and 3 months after the education using the microbiota knowledge test, and statistical analysis was conducted.
Conclusions:
Through peer education, areas of confusion experienced by students regarding microbiota were gamified, resolved through mutual knowledge exchange, and awareness of microbiota was raised.
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