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DNA aptamer-based sensitive electrochemical biosensor for NAD(H) detection.

Wenfei Guo1, Haiyuan Wang2, Zhaoyang Wang2

  • 1Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan, 410082, China; Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), The Chinese Academy of Sciences, Hangzhou, Zhejiang, 310022, China.

Biosensors & Bioelectronics
|November 29, 2024
PubMed
Summary
This summary is machine-generated.

A new aptamer-based biosensor accurately detects nicotinamide-adenine dinucleotide (NAD(H)), a key molecule in cellular metabolism. This tool reveals metabolic differences in cancer cells and aging tissues.

Keywords:
AptamerDNA nanostructureElectrochemical biosensorMetabolic differenceNAD(H)

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Nicotinamide-adenine dinucleotide (NAD(H)) is crucial for cellular metabolism and understanding its levels is vital for disease research.
  • Developing specific probes and sensitive biosensors for NAD(H) detection remains a significant scientific challenge.

Purpose of the Study:

  • To develop a highly selective and sensitive electrochemical biosensor for accurate NAD(H) detection.
  • To screen and identify aptamers with specific binding affinity for NAD(H).

Main Methods:

  • Screening of aptamers to identify specific NAD(H) binders, exemplified by NAD3-1a with micromolar affinity.
  • Integration of the selected aptamer with a tetrahedral DNA nanostructure to create an electrochemical biosensor.
  • Validation of the biosensor's performance, including its linear detection range, stability, and reproducibility.

Main Results:

  • The developed aptamer-based biosensor demonstrated high selectivity and sensitivity for NAD(H) detection within a linear range of 10⁻¹² to 10⁻⁷ M.
  • The biosensor exhibited excellent stability and reproducibility in measurements.
  • Significant variations in NAD(H) levels were observed between normal and tumor cells.
  • A notable reduction in NAD(H) levels was detected in the skeletal muscle tissues of aged mice.

Conclusions:

  • The aptamer-based electrochemical biosensor offers a powerful tool for precise NAD(H) quantification.
  • This biosensor has the potential to significantly advance the understanding of metabolic dysregulation in various physiological and pathological conditions.
  • The findings underscore the utility of aptamer-DNA nanostructure conjugates in developing advanced biosensing platforms for critical biomolecules.