Bimetallic CuMn nanozyme-enzyme microsystem for efficient dimethyl phthalate degradation

Area of Science:

• Environmental Science
• Materials Science
• Biotechnology

Background:

• Nanozyme-enzyme microsystems offer enhanced catalytic stability for pollutant treatment.
• Developing robust systems is crucial for addressing complex aquatic contamination.

Purpose of the Study:

• To construct and evaluate a carboxyl-functionalized CuMn bimetallic nanozyme-enzyme microsystem (CMAC@Lipase).
• To assess its catalytic activity, stability, and efficiency in degrading pollutants under alkaline conditions.

Main Methods:

• Synthesized CMAC@Lipase by combining copper-manganese aminoclays (CMAC) with lipase.
• Investigated laccase-like activity via CuMn electron transfer and lipase stabilization.
• Evaluated the degradation of p-nitrophenyl phosphate (p-NPP) and dimethyl phthalate (DMP) under alkaline conditions.

Main Results:

• CMAC@Lipase demonstrated laccase-like activity and enhanced lipase stability.
• Successfully catalyzed p-NPP hydrolysis and reduction to p-AP within 30 minutes.
• Degraded 72.8% of dimethyl phthalate (DMP) within 48 hours, maintaining 53.5% degradation after 10 reuse cycles.

Conclusions:

• The developed CMAC@Lipase microsystem presents a novel strategy for nanozyme-enzyme design.
• This system offers a promising approach for efficient treatment of contaminants in real aquatic environments.