Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Association of SARS-CoV-2 infection with incident diabetes among U.S. Veterans in a prospective longitudinal cohort.

PloS one·2026

Same author

Assessment of a membrane filter coated with hygroscopic glycerol for improved recovery of airborne viable bacteriophage MS2.

Aerosol and air quality research·2026

Same author

Concentrating viable airborne pathogens using a virtual impactor with a compact water-based condensation air sampler.

Aerosol science and technology : the journal of the American Association for Aerosol Research·2024

Same author

Effects of Pitavastatin on COVID-19 Incidence and Seriousness Among a Global Cohort of People With HIV.

Open forum infectious diseases·2024

Same author

Condyloma lata in an HIV patient with secondary syphilis masquerading as anal condyloma-a case report.

International journal of colorectal disease·2024

Same author

Sample preparation and detection methods in point-of-care devices towards future at-home testing.

Lab on a chip·2024

Search research articles

Related Experiment Video

Updated: Jun 6, 2025

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

A handheld HIV detection platform using paper-based sample preparation and real-time isothermal amplification.

George Adedokun¹, Gurjit Sidhu², Morteza Alipanah¹

¹Interdisciplinary Microsystems Group, Department of Mechanical & Aerospace Engineering, University of Florida, Gainesville, FL, 32611, USA.

Microsystems & Nanoengineering

|November 29, 2024

Summary

A new, low-cost platform enables early human immunodeficiency virus (HIV) diagnosis at the point-of-care. This portable device integrates nucleic acid extraction and amplification for rapid HIV detection without lab infrastructure.

More Related Videos

Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection

Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection

Published on: January 26, 2024

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Related Experiment Videos

Last Updated: Jun 6, 2025

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection

Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection

Published on: January 26, 2024

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Area of Science:

Biomedical Engineering
Infectious Disease Diagnostics
Point-of-Care Testing

Background:

Accurate human immunodeficiency virus (HIV) diagnosis is crucial for effective antiretroviral therapy (ART) and infection control.
Conventional nucleic acid tests for HIV require specialized laboratory equipment and trained staff, limiting their use in resource-limited settings and at the point-of-care (POC).

Purpose of the Study:

To develop an affordable, integrated platform for POC HIV testing.
To enable rapid and accurate detection of HIV nucleic acid without reliance on laboratory infrastructure.

Main Methods:

Development of a portable, battery-powered device integrating paper-based nucleic acid extraction and reverse transcription loop-mediated isothermal amplification (RT-LAMP).
Real-time detection assay designed for POC use, requiring no external power outlets.
Validation using spiked and clinical samples, compared against standard polymerase chain reaction (PCR) assays.

Main Results:

The platform achieved a detection limit of 30 copies/mL HIV RNA in serum.
Demonstrated high sensitivity with 4 copies/reaction using 50 μL serum.
Showed no cross-reactivity with hepatitis C virus (HCV) and was validated against PCR methods.

Conclusions:

The developed integrated platform is feasible for POC HIV testing.
This technology offers a low-cost, accessible solution for early HIV diagnosis in diverse settings.
The system bypasses the need for traditional laboratory infrastructure, enhancing POC diagnostic capabilities.