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Updated: Apr 13, 2026
Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
Published on: June 27, 2013
Quantitatively Analyzing the Morphological Growth Dynamics of Bipolar and Non-bipolar Spindles Using Xenopus Egg
Akihiro Tanaka1, Tatsuya Fukuyama2, Yusuke T Maeda2
1Department of Chromosome Science, National Institute of Genetics, Shizuoka, Japan.
None:
The bipolar shape of the microtubule-based spindle is a pivotal morphological phenotype for accurate chromosome segregation during cell division. However, existing descriptions of spindle morphogenesis remain largely qualitative. Here, we introduce a method that provides a quantitative description of the morphological growth dynamics of spindles using Xenopus egg cytoplasmic extract and a computational image analysis pipeline. Our method allows for determining the characteristic growth dynamics of bipolar and non-bipolar spindles, comparing their spatiotemporal differences and changes in response to physical and biochemical perturbations.
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