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Updated: Jun 6, 2025
Optimized PCR-based Detection of Mycoplasma
Published on: June 20, 2011
Mpox Specimen Collection, Storage, Preparation, and Laboratory Diagnostics by PCR
David Lowe1, Whitni Davidson2, Kimberly Wilkins2
1National Center for Immunizations and Respiratory Diseases, Coronavirus and Other Respiratory Viruses Laboratory Branch, U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA. nqu9@cdc.gov.
Abstract:
During the 2022 and ongoing mpox outbreak, PCR diagnostics were already within the Laboratory Response Network (LRN) laboratories and additionally rapidly deployed to detect monkeypox (mpox) virus in patients and aid in accurate diagnosis. This accuracy is dependent on proper collection, shipment, and adherence to PCR procedure. This book chapter discusses effective collection, storage, and transportation for a real-time PCR to detect monkeypox virus nucleic acid.
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