Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Next-Generation Barcoding for Single-Cell Omics.

Analytical chemistry·2025

Same author

OmicsCam Enables Trimodal Profiling of Mitochondrial Genome Editing.

Analytical chemistry·2025

Same author

Expanding the Dimensions of Single-Cell Multi-Omics To Include Mitochondrial DNA through Fixation and Permeabilization.

Analytical chemistry·2025

Same author

Harnessing HetHydrogel: A Universal Platform to Dropletize Single-Cell Multiomics.

Small methods·2024

Same author

CSBF/C10orf99, a novel potential cytokine, inhibits colon cancer cell growth through inducing G1 arrest.

Scientific reports·2014

Same author

V‑set and transmembrane domain‑containing 1 is silenced in human hematopoietic malignancy cell lines with promoter methylation and has inhibitory effects on cell growth.

Molecular medicine reports·2014

Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026

Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026

Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026

Same journal

TopoStitcher: A Geometric-Topological Structure-Guided Stitching Framework for Single-Molecule Localization Microscopy.

Analytical chemistry·2026

Same journal

Noninvasive SERS Immunosensing of Tyrosinase for Melanoma Monitoring via Microneedle Sampling Integrated with Satellite-Structured Bifunctional Nanozymes.

Analytical chemistry·2026

Same journal

Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection.

Analytical chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 20, 2026

Evaluation of Cancer Stem Cell Migration Using Compartmentalizing Microfluidic Devices and Live Cell Imaging

Evaluation of Cancer Stem Cell Migration Using Compartmentalizing Microfluidic Devices and Live Cell Imaging

Published on: December 23, 2011

Permeability-Engineered Compartmentalization System Promises Next-Generation Single-Cell Analysis.

Ting Li¹, Zhenglong Gu^2,3, Guoqiang Zhou^4,2

¹Human Phenome Institute, Fudan University, Shanghai 200438, China.

Analytical Chemistry

|December 2, 2024

Summary

This summary is machine-generated.

Permeability-engineered compartmentalization systems overcome limitations of traditional single-cell analysis platforms. These advanced systems enable controlled material exchange for improved multiomics and biochemical assays.

More Related Videos

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Sealable Femtoliter Chamber Arrays for Cell-free Biology

Sealable Femtoliter Chamber Arrays for Cell-free Biology

Published on: March 11, 2015

Related Experiment Videos

Last Updated: Jun 20, 2026

Evaluation of Cancer Stem Cell Migration Using Compartmentalizing Microfluidic Devices and Live Cell Imaging

Evaluation of Cancer Stem Cell Migration Using Compartmentalizing Microfluidic Devices and Live Cell Imaging

Published on: December 23, 2011

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Sealable Femtoliter Chamber Arrays for Cell-free Biology

Sealable Femtoliter Chamber Arrays for Cell-free Biology

Published on: March 11, 2015

Area of Science:

Biomedical Research
Single-Cell Analysis
Compartmentalization Technologies

Background:

Microplates and microfluidic droplets are foundational for single-cell analysis but have limitations.
Microplates offer open systems for reagent handling but lack nano-scale compartmentalization.
Microfluidic droplets provide nano-scale compartments but hinder material exchange.

Purpose of the Study:

To introduce permeability-engineered compartmentalization systems for single-cell analysis.
To address the limitations of existing single-cell analysis platforms.
To explore advancements and applications of novel compartmentalization strategies.

Main Methods:

Review of recent innovations in permeability-engineered compartmentalization.
Discussion of underlying principles of controllable material exchange.
Exploration of applications in single-cell multiomics and biochemical assays.

Main Results:

Permeability-engineered systems offer controllable material exchange, overcoming droplet isolation issues.
These systems facilitate complex multistep biochemical reactions.
Advancements support single-cell multiomics and nonsequencing applications.

Conclusions:

Permeability-engineered compartmentalization represents a significant advancement in single-cell analysis.
These systems enhance the capabilities of ex vivo research and multiomics.
Future applications promise to expand the scope of single-cell investigations.