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A Low-Cost Microfluidic Device For the On-Line Counting of Microparticle/Bacteria.

Zhen-Yu Xun¹, Lv Liu¹, Bai-Chuan Zhang^1,2

¹School of Mechanical Engineering, Xi'an JiaoTong University, Xi'an, Shaanxi, People's Republic of China.

Electrophoresis

|December 3, 2024

Summary

A new low-cost microfluidic device efficiently counts microparticles and bacteria in liquids. This technology enhances accuracy for food safety and biomedical applications.

Keywords:

bacteria counting focusing microfluidic device microparticle

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Area of Science:

Biomedical Engineering
Microfluidics
Analytical Chemistry

Background:

Accurate on-line counting of microparticles and bacteria is crucial for food safety and biomedical diagnostics.
Existing methods can be costly and complex, limiting widespread application.

Purpose of the Study:

To develop a low-cost, efficient microfluidic device for real-time microparticle and bacteria counting.
To integrate elastic focusing and light resistance detection for improved accuracy.

Main Methods:

A microfluidic device with a gradually contracted channel and viscoelastic fluid was designed for elastic focusing.
A simple optical detection system using a laser pointer, lens, diaphragm, and photodiode was integrated.
Experiments investigated the effects of flow rate, particle size, fluid properties, and channel structure on particle focusing.

Main Results:

Efficient elastic focusing of particles/bacteria was achieved, aligning them centrally.
The integrated device demonstrated accurate counting of various microparticle sizes and Bifidobacterium.
The device's performance was validated through experimental investigations.

Conclusions:

The developed microfluidic device offers a simple, low-cost, and efficient solution for on-line particle and bacteria counting.
This technology has significant potential for commercial applications in food safety and clinical diagnostics.
The device is suitable for real-time monitoring of microplastic contamination and bacterial colony-forming units (CFU).